The interaction between endogenous DNMT3A and CEBPA proteins was confirmed in K562 and HL-60 cells via IP coupled with Western blotting (fig. S1C). To further confirm the specificity of endogenous co-IP, we found that CEBPA was immunoprecipitated by the DNMT3A antibody in wild-type HL-60 cells, but not in DNMT3A -KO cells ( Fig. 1F ). Sphingosine levels appeared to increase modestly (Additional file 1: Figure S1C and D), suggesting that the balance between sphingosine phosphorylation and dephosphorylation is affected in AD pathogenesis. ... Disciplines of Medicine and Pathology, Sydney Medical School, The University of Sydney, Sydney, 2006, NSW, Australia. Jillian Kril.

prior to reverse transcription (Fig. S1C). Here, we observed loss of signal from cytoplasmic regions of cells (Fig. S1C; vim-FAM), Figure 1. (A) entire method is schematically presented in (A), where magnified view of cells is depicted. Different stages of this method involving conversion of RNA into cDNA by in situ.